Moreover, domestic pig breeding started relatively late, and most of the core provenances of lean pigs rely on imports. Certain problems have been found in Chinese pork breeds, such as a low lean meat rate and a lack of lean pig breeds to conduct breed improvements. At present, the fat content of pork in China is high, but the amount of muscle is insufficient. The world’s average supply of protein of animal origin (g/cap/day) has increased from 26.8 g in 2000 to 31.6 g today (FAO). With the development of society and improvements in people’s economic levels, consumers are paying more attention to the quality of pork. Pork plays an important role in China’s consumption and daily diet and a vital role in the national economy. According to FAO statistics, China’s pork production ranked first in the world in 2020. China is one of the leading countries in pork consumption. Pork is the third primary source of animal protein for human consumption and is still the most important meat production and marketing variety in the world (FAO). Our results explored the mechanism and expression profile of mRNAs and lncRNAs of ZBED6 gene knockout on myocardium tissue development, mining the key candidate genes in that process like IGF2. Moreover, IGF2 was the direct target gene of ZBED6 by ChIP-PCR experiment. The functional annotation analysis based on the Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) databases revealed two GO items related to muscle development by the cis target genes of DELs. A total of 248 differentially expressed genes (DEGs) and 209 differentially expressed lncRNAs (DELs) were detected, and 105 potential cis target genes of DELs were identified. To investigate the function of ZBED6 in the cardiac development of pigs, we constructed the expression profiles of mRNAs and lncRNAs in myocardial tissue obtained from Bama Xiang pigs in the ZBED6 knockout group ( ZBED6-KO) and the wild-type group ( ZBED6-WT). Emerging evidence suggests that long noncoding RNAs (lncRNAs) can regulate genes at the epigenetic, transcriptional, and posttranscriptional levels and play an important role in the development of eukaryotes. However, its effect on the growth and development of the heart is still unknown. The ZBED6 gene is a transcription factor that regulates the expression of IGF2 and affects muscle growth and development.
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